Contact and fumigant toxicities of calamusenone isolated from Acorus gramineus rhizome against adults of Sitophilus zeamais and Rhizopertha dominica

Calamusenone (3,8-dimethyl-5-(1-methylethylidene)-1,2,3,4,5,6,7,8-octahydroazulene-6- one, C15H22O) from Acorus gramineus Soland rhizome was tested in the laboratory for its insecticidal activities against adults of Sitophilus zeamais Motsch. and Rhizopertha dominica (Fab.), using dry film contact and fumigation methods. Responses varied with insect species, dosage and exposure time. In the dry film contact experiment, the highest insecticidal effects of calamusenone against S. zeamais and R. dominica adults were produced at 170.32 [mu]g/cm2 after treatment for 72 h, with 96.2% and 98.7% mortalities, respectively. The median lethal concentration (LC50) (72 h) values of calamusenone against S. zeamais and R. dominica adults were 67.00 [mu]g/cm2 and 77.30 [mu]g/cm2, respectively. As a potential fumigant, calamusenone showed moderate insecticidal effect on the adults of S. zeamais and R. dominica in fumigation experiment, with their LC50 (120 h) values of 125.71 [mu]L/L and 93.64 [mu]L/L respectively. Calamusenone isolated from A. gramineus rhizome showed promise as a novel pesticide candidate for stored-product pest control.