TY - JOUR T1 - Mitochondrial DNA phylogenetic analysis of the genus Laparocerus (Coleoptera, Curculionidae, Entiminae). I. The Madeiran clade JF - Zoologica Scripta Y1 - 2008 A1 - Machado, Antonio A1 - Lopez, Mercedes A1 - Almeida, Teresa A1 - Hernandez, Mariano SP - 415 EP - 427 KW - Atlantis KW - Cyphoscelis KW - Drouetius KW - Laparocerus KW - Laparocerus (Lichenophagus) KW - Laparocerus chaoensis KW - Laparocerus morio KW - Lichenophagus AB - Laparocerus are plant-chewing flightless weevils distributed on oceanic islands in Macaronesia, with a single species in Morocco. The genus has a complicated taxonomic history with several subgenera described. To assist in a taxonomical revision of the group, a molecular study was undertaken. In this first contribution, the species from the Azores and Madeira archipelagos are studied together with representatives of subgenera from the Canary Islands and the single known continental species (46 OTUs). Phylogenetic analyses are based on sequence data from mitochondrial cytochrome oxidase II (COII) and the ribosomal 16S ribosomal RNA (16S rRNA) genes (combined data set 1023 bp), with additional data from the nuclear elongation factor 1[alpha] (EF-1[alpha]) for some selected species. Maximum likelihood (ML) and Bayesian analyses show that all Madeiran species are monophyletic and form a monophyletic group with the Afro-Canarian samples. Species of the genus Lichenophagus appear within the Madeiran and Canarian Laparocerus clades, but separated in accordance with their respective island origin. Thus, Lichenophagus is here restricted to Madeiran species and proposed as subgenus (status novo) of Laparocerus. Conversely, the Laparocerus subgenus Drouetius from the Azores is revealed to be a separate and distant outgroup, in agreement with its morphological distinctiveness, deserving an independent genus status. Internal relationships within the Madeiran clade are discussed and compared with morphologically defined species groups. The Madeiran monotypic subgenus Cyphoscelis is not supported by the genetic data and synonymized (nov. syn.) with Laparocerus. Subgenera Laparocerus and Atlantis prove to be polyphyletic. Consequently a restriction to monophyletic and morphologically congruent clades is proposed. A cryptic species vicariant of Laparocerus morio was detected and recognized as L. chaoensis, status novo. Other cases of discrepancy between the genetic results and the traditional taxonomy are discussed in detail in the light of mitochondrial introgression, incomplete lineage sorting or poor taxonomy hypotheses. VL - 37 SN - 0300-3256 ER - TY - JOUR T1 - The genus Drouetius Mequignon, 1942, stat. prom., from the Azores (Coleoptera, Curculionidae, Entiminae) JF - Graellsia Y1 - 2009 A1 - Machado, A. SP - 19 EP - 46 KW - Drouetius KW - Drouetius azoricus KW - Drouetius azoricus nitens KW - Drouetius azoricus paralellirostris KW - Drouetius azoricus separandus KW - Drouetius borgesi KW - Drouetius borgesi centralis KW - Drouetius borgesi sanctimichaelis KW - Drouetius oceanicus KW - Drouetius oceanicus tristis KW - Laparocerus KW - Peritelus AB - Drouetius Mequignon, 1942, was originally established as a monotypical subgenus of Laparocerus Schonherr, 1834, and is here upgraded to genus (stat. prom.) based on morphological and molecular data. The genus and D. azoricus are redescribed, and D. azoricus nitens n. ssp., D. azoricus paralellirostris n. ssp. D. azoricus separandus n. ssp., D oceanicus n. sp., D. oceanicus tristis n. ssp., D. borgesi n. sp., D. borgesi sanctimichaelis n. ssp., and D. borgesi centralis n. ssp. are described. The different form of the transfer apparatus of the aedeagus has been used as criterion for aggregating the island populations into a single species. A tentative phylogenetic test based on partial sequences of the mitochondrial gene 16SrRNA from representative species of several tribes of Entiminae, points to a closer relationship between Drouetius and Peritelus, than with Brachyderes or other genera. Finally, a key for the identification of the species and subspecies is provided, as well as comments on their ecology and distribution in the Azores archipelago, where Drouetius is endemic. VL - 65 SN - 0367-5041 ER - TY - JOUR T1 - Mitochondrial DNA phylogenetic analysis of the genus Laparocerus (Coleoptera, Curculionidae, Entiminae). I. The Madeiran clade JF - Zoologica Scripta Y1 - 2008 A1 - Machado, Antonio A1 - Lopez, Mercedes A1 - Almeida, Teresa A1 - Hernandez, Mariano SP - 415 EP - 427 KW - Atlantis KW - Cyphoscelis KW - Drouetius KW - Laparocerus KW - Laparocerus (Lichenophagus) KW - Laparocerus chaoensis KW - Laparocerus morio KW - Lichenophagus AB - Laparocerus are plant-chewing flightless weevils distributed on oceanic islands in Macaronesia, with a single species in Morocco. The genus has a complicated taxonomic history with several subgenera described. To assist in a taxonomical revision of the group, a molecular study was undertaken. In this first contribution, the species from the Azores and Madeira archipelagos are studied together with representatives of subgenera from the Canary Islands and the single known continental species (46 OTUs). Phylogenetic analyses are based on sequence data from mitochondrial cytochrome oxidase II (COII) and the ribosomal 16S ribosomal RNA (16S rRNA) genes (combined data set 1023 bp), with additional data from the nuclear elongation factor 1[alpha] (EF-1[alpha]) for some selected species. Maximum likelihood (ML) and Bayesian analyses show that all Madeiran species are monophyletic and form a monophyletic group with the Afro-Canarian samples. Species of the genus Lichenophagus appear within the Madeiran and Canarian Laparocerus clades, but separated in accordance with their respective island origin. Thus, Lichenophagus is here restricted to Madeiran species and proposed as subgenus (status novo) of Laparocerus. Conversely, the Laparocerus subgenus Drouetius from the Azores is revealed to be a separate and distant outgroup, in agreement with its morphological distinctiveness, deserving an independent genus status. Internal relationships within the Madeiran clade are discussed and compared with morphologically defined species groups. The Madeiran monotypic subgenus Cyphoscelis is not supported by the genetic data and synonymized (nov. syn.) with Laparocerus. Subgenera Laparocerus and Atlantis prove to be polyphyletic. Consequently a restriction to monophyletic and morphologically congruent clades is proposed. A cryptic species vicariant of Laparocerus morio was detected and recognized as L. chaoensis, status novo. Other cases of discrepancy between the genetic results and the traditional taxonomy are discussed in detail in the light of mitochondrial introgression, incomplete lineage sorting or poor taxonomy hypotheses. VL - 37 SN - 0300-3256 ER - TY - JOUR T1 - The genus Drouetius Mequignon, 1942, stat. prom., from the Azores (Coleoptera, Curculionidae, Entiminae) JF - Graellsia Y1 - 2009 A1 - Machado, A. SP - 19 EP - 46 KW - Drouetius KW - Drouetius azoricus KW - Drouetius azoricus nitens KW - Drouetius azoricus paralellirostris KW - Drouetius azoricus separandus KW - Drouetius borgesi KW - Drouetius borgesi centralis KW - Drouetius borgesi sanctimichaelis KW - Drouetius oceanicus KW - Drouetius oceanicus tristis KW - Laparocerus KW - Peritelus AB - Drouetius Mequignon, 1942, was originally established as a monotypical subgenus of Laparocerus Schonherr, 1834, and is here upgraded to genus (stat. prom.) based on morphological and molecular data. The genus and D. azoricus are redescribed, and D. azoricus nitens n. ssp., D. azoricus paralellirostris n. ssp. D. azoricus separandus n. ssp., D oceanicus n. sp., D. oceanicus tristis n. ssp., D. borgesi n. sp., D. borgesi sanctimichaelis n. ssp., and D. borgesi centralis n. ssp. are described. The different form of the transfer apparatus of the aedeagus has been used as criterion for aggregating the island populations into a single species. A tentative phylogenetic test based on partial sequences of the mitochondrial gene 16SrRNA from representative species of several tribes of Entiminae, points to a closer relationship between Drouetius and Peritelus, than with Brachyderes or other genera. Finally, a key for the identification of the species and subspecies is provided, as well as comments on their ecology and distribution in the Azores archipelago, where Drouetius is endemic. VL - 65 SN - 0367-5041 ER -