TY - JOUR T1 - Exploring diversity in cryptorhynchine weevils (Coleoptera) using distance-, character- and tree-based species delineation JF - Molecular Phylogenetics and Evolution Y1 - 2012 A1 - Astrin, Jonas J. A1 - Stueben, Peter E. A1 - Misof, Bernhard A1 - Waegele, J. Wolfgang A1 - Gimnich, France A1 - Raupach, Michael J. A1 - Ahrens, Dirk SP - 1 EP - 14 KW - Cryptorhynchinae AB - Species boundaries are studied in a group of beetles, the western Palaearctic Cryptorhynchinae. We test for congruence of 'traditionally' identified morphospecies with species inferred through parsimony networks, distance-based clustering and the ultrametric tree-based generalized mixed yule-coalescent (GMYC) approach. For that purpose, we sequenced two variable fragments of mitochondrial DNA (CO1 and 16S) for a total of 791 specimens in 217 species of Cryptorhynchinae. Parsimony networks, morphology-calibrated distance clusters and the different tree-based species inferences all achieved low congruence with morphospecies, at best 60%. Although the degree of match with morphospecies was often similar for the different approaches, the composition of clusters partially varied. A barcoding gap was absent in morphospecies-oriented distances as well as for GMYC species clusters. This demonstrates that not only erroneous taxonomic assignments, incomplete lineage sorting, hybridization, or insufficient sampling can compromise distance-based identification, but also differences in speciation rates and uneven tree structure. The initially low match between morphospecies and the different molecular species delineation methods in this case study shows the necessity of combining the output of various methods in an integrative approach. Thereby we obtain an idea about the reliability of the different results and signals, which enables us to fine-tune sampling, delineation technique and data collection, and to identify species that require taxonomic revision. (C) 2011 Elsevier Inc. All rights reserved. VL - 63 SN - 1055-7903 ER - TY - BOOK T1 - Molecular phylogeny of the treeclimbers T2 - Treeclimbers: Dendroacalles, Silvacalles and Lauriacalles of the Canary Islands Y1 - 2009 A1 - Astrin, Jonas J. ED - Stueben, Peter E. ED - Fabian, Klaus ED - Astrin, Jonas J. SP - 38 EP - 41 JF - Treeclimbers: Dendroacalles, Silvacalles and Lauriacalles of the Canary Islands SN - 978-3-00-028089-4 ER - TY - JOUR T1 - Molecular phylogeny in 'nano-weevils': a description of a new subgenus Nanoacalles and two new species of Calacalles from the Macaronesian Islands (Curculionidae: Cryptorhynchinae) JF - Zootaxa Y1 - 2009 A1 - Astrin, Jonas J. A1 - Stuben, Peter E. SP - 51 EP - 67 VL - 2300 ER - TY - JOUR T1 - Molecular phylogeny in endemic weevils: revision of the genera of Macaronesian Cryptorhynchinae (Coleoptera: Curculionidae) JF - Zoological Journal of the Linnean Society Y1 - 2010 A1 - Stüben, P.E. A1 - Astrin, Jonas J. SP - 40 EP - 87 AB - A molecular phylogeny and lineage age estimates are presented for the Macaronesian representatives of the weevil subfamily Cryptorhynchinae, using two mitochondrial genes (cytochrome c oxidase subunit 1 and 16S). The Bayesian reconstruction is supplemented by observations on morphology, ecology, and reproductive biology. The present study often corroborates the groups previously outlined in higher-level informal taxonomies. These and further groups are now assigned new taxonomic status. The following genera and subgenera are described (formerly Acalles): Aeoniacalles gen. nov., Canariacalles gen. nov., Ficusacalles gen. nov., Madeiracalles gen. nov., Silvacalles gen. nov. (with Tolpiacalles subgen. nov., Tagasastacalles subgen. nov.), Sonchiacalles gen. nov., Echiumacalles gen. nov. (monotypic), Lauriacalles gen. nov. (monotypic), and Pseudodichromacalles gen. nov. (monotypic; formerly Dichromacalles). For the western Palaearctic genus Acalles Schoenherr, 1825 the first subgenus Origoacalles subgen. nov. is described and for the genus Onyxacalles Stüben, 1999 the first subgenus Araneacalles subgen. nov.; Paratorneuma Roudier 1956 resyn. Except for one species of Acalles (Origoacalles), all of these new higher taxa are endemic to the Macaronesian Islands. All new taxa are presented, together with their host plants and further data, in a synoptic tabular overview. Based on the results of our phylogenetic analysis, we advocate the hypothesis that the evolution of the species in the new genera (of which most group into a ‘Macaronesian clade’) began in the comparatively arid succulent bush zone and that the shady and humid laurel forest of the thermo-Canarian and thermo-Madeiran zone was entered much later. Our reconstruction implies that the Canarian and Madeiran archipelagos were colonized by Cryptorhynchinae at least seven times from the continent but saw only one considerable adaptive radiation. It also becomes apparent that it is the ancestor species of the genus Canariacalles – and not Pseudodichromacalles – that features a close connection to the south-western European and north-western African species of Dichromacalles s.s. Finally, a key is presented for all genera and subgenera of the Macaronesian Cryptorhynchinae. VL - 160 ER - TY - BOOK T1 - Treeclimbers: Dendroacalles, Silvacalles and Lauriacalles of the Canary Islands (Coleoptera: Cryptorhynchinae) Y1 - 2009 A1 - Stueben, Peter E. A1 - Fabian, K. A1 - Astrin, Jonas J. KW - Dendroacalles KW - Lauriacalles KW - Silvacalles KW - Silviacalles (Tolpiacalles) AB - The TreeClimbers s.l. comprise the exclusively tree-living Cryptorhynchinae (Coleoptera: Curculionidae) that are found mainly in the Canary Islands. Klaus Fabian was asked to portray these colourful and bizarrely-bristled acrobats of the higher strata and canopies of the Canarian and - only two species- Madeiran laurel forest. At the beginning there was 'curiousness (if it might succeed) and fascination', because for the coleopterologist who was used to shifting the small number of Central European species of Acalles from the leaf litter of the native beech and oak forest, it was a big surprise to begin the subtle hunt in the moist, shady and evergreen forest of the thermo-canarian belt. This was more than ten years ago and it required the development of new techniques like beating with long stakes into huge beating trays to explore the Cryptorhynchinae fauna of the canopies. So-called, 'canopy fogging' with natural pyrethrum, a method which we applied on the Canary Islands in autumn 2008 for the first time, seemed much more promising and effective. Where the forest was not felled in the past - as in the national park of Garojonay on La Gomera - old and huge trees are located between large and impressive roots, evoking an unearthly atmosphere during the night. This is the real homeland of the TreeClimbers. In such habitats the specimens can be beaten in large numbers from the metres-long shoots growing from the base of the tree and the roots of the Lauraceae. From the phylogenetical point of view the TreeClimbers are highly derived'taxa' and, ecologically, these groups are extreme tree-specialists which colonized a very old habitat, the 'laurisilva', only in the recent geological history. way. In this illustrated book we want to build a bridge between art, morphology and molecular biology [long dash] an attempt to understand entomology as art and science again ... PB - Curculio Institute CY - Moenchengladbach SN - 978-3-00-028089-4 ER - TY - JOUR T1 - Molecular phylogeny of Echinodera and Ruteria (Coleoptera: Curculionidae: Cryptorhynchinae) and the parallel speciation of Canary island weevils along replicate environmental gradients JF - Invertebrate Systematics Y1 - 2010 A1 - Astrin, Jonas J. A1 - Stueben, Peter E. SP - 434 EP - 455 KW - Echinodera bargouensis KW - Echinodera gomerensis KW - Ruteria bellieri AB - A molecular phylogeny for the western Palaearctic weevil genus Echinodera Wollaston, 1863 and the former genus Ruteria Roudier, 1954 is presented, combining two mitochondrial genes (CO1 and 16S) in a Bayesian analysis. Special consideration is given to the species of Echinodera from the Canary Islands. Between islands, these are represented by multiple vicariant species that have undergone parallel speciation along replicate environmental gradients on the respective islands. Based on the phylogenetic tree and further data, a number of taxonomic changes is presented: two new species are described, Echinodera montana, sp. nov. from the Canaries (Fuerteventura) and Echinodera bargouensis, sp. nov. from Tunisia. Five species are declared to be synonyms: Echinodera gomerensis Stuben, 2000, syn. nov. = Echinodera praedicta Germann & Stuben, 2006, syn. nov. = Echinodera pseudohystrix Stuben, 2000; Ruteria bellieri epirica Wolf, 2001, syn. nov. = Echinodera tyrrhenica Caldara, 1978, syn. nov. = Acalles bellieri Reiche, 1860; Echindera troodosi Wolf, 2010, syn. nov. = Echinodera cyprica Stuben, 2010. The subgenus Echinodera (Dieckmannia) Stuben, 1998 is a synonym of Echinodera s. str. The genus Ruteria is again declared a subgenus of Echinodera: Echinodera (Ruteria) Roudier, 1954 stat. rev. Two species are transferred to a different subgenus: Echinodera (Ruteria) incognita (Hoffmann, 1956) and Echinodera (Ruteria) cognita Stuben, 2006 (both formerly Echinodera s. str.). VL - 24 SN - 1445-5226 ER - TY - JOUR T1 - New insights from biogeography, morphology and molecular biology: the species status of Acalles temperei Pericart, 1987 and Kyklioacalles navieresi (Boheman, 1837) (Curculionidae: Cryptorhynchinae) JF - Weevil News Y1 - 2006 A1 - Stueben, Peter E. A1 - Astrin, Jonas J. SP - Unpaginated KW - Acalles parvulus KW - Acalles temperei KW - Kyklioacalles navieresi KW - Kyklioacalles roboris AB - Biogeographical and morphological arguments are presented that suggest the following synonymization: Acalles parvulus Boheman, 1837 = Acalles temperei Pericart, 1987. Comparing the aedeagi along a transect from Mont Pilat (Dep. Loire) to Mont Saint-Martin North of Grenoble (Dep. Isere), a cline becomes apparent that raises doubts about the species status of Acalles temperei. These results, obtained by comparative phenotypic examination, are corroborated in a preliminary set of molecular genetic analyses. The latter either hint the existence of a hybrid zone or indicate that Acalles parvulus and Acalles temperei constitute a single, geographically structured species. The molecular results for Kyklioacalles roboris (Curtis, 1834) and the recently resynonymized species Kyklioacalles navieresi (Boheman, 1837) are very different. DNA sequence analysis of the mitochondrial CO1 and 16S genes and of the nuclear 28S gene showed that Kyklioacalles navieresi and Kyklioacalles roboris likely represent two individual, but closely related species. VL - 33 SN - 1615-3472 UR - http://www.curci.de/Inhalt.htm ER - TY - JOUR T1 - Molecular phylogeny in 'nano-weevils': description of a new subgenus Nanoacalles and two new species of Calacalles from the Macaronesian Islands (Curculionidae: Cryptorhynchinae) JF - Zootaxa Y1 - 2009 A1 - Astrin, Jonas J. A1 - Stueben, Peter E. SP - 51 EP - 67 KW - Calacalles KW - Calacalles hermigua KW - Calacalles nataliae AB - A molecular phylogeny for the eastern Atlantic weevil genus Calacalles Peyerimhoff, 1925 is presented, using two mitochondrial genes (CO1 and 16S). Based on a phylogenetic (Bayesian) analysis, we propose the following taxonomic amendments: the new subgenus Nanoacalles subg. n. is described and two new species, Calacalles hermigua sp. n. and Calacalles nataliae sp. n., are described from the Canarian island La Gomera and distinguished from other species of the genus. Calacalles palmensis (Roudier, 1954) (formerly Acalles wollastoni palmensis Roudier, 1954), which is illustrated here for the first time (habitus and aedeagus), is not a synonym of Acalles seticollis Wollaston, 1864, but a valid taxon. Calacalles subcarinatus (Israelson, 1984) from the Azores is assigned to the subgenus Crateracalles Stuben 2004. We also present a catalogue of all known Calacalles species. VL - 2300 SN - 1175-5326 UR - http://www.mapress.com/zootaxa ER - TY - JOUR T1 - Phylogeny in cryptic weevils: molecules, morphology and new genera of western Palaearctic Cryptorhynchinae (Coleoptera: Curculionidae) JF - Invertebrate Systematics Y1 - 2008 A1 - Astrin, Jonas J. A1 - Stueben, Peter E. SP - 503 EP - 522 KW - Coloracalles KW - Coloracalles humerosus KW - Cryptorhynchinae KW - Elliptacalles KW - Elliptacalles longus KW - Kyklioacalles KW - Montanacalles KW - Montanacalles nevadaensis AB - A phylogeny is presented for the western Palaearctic representatives of the weevil subfamily Cryptorhynchinae using a combination of phenotypic and genotypic characters. This phylogeny is the first for the extremely species-rich Cryptorhynchinae to use molecular data (mitochondrial CO1 and 16S as well as nuclear ribosomal 28S). The results of this study show the need for molecular tools within this morphologically cryptic group of weevils and provide a scaffold based on which genus assignment can be tested. The present study mostly corroborates the current subdivision into genera (but many of the subgeneric groups are questioned). Three new genera are described: Montanacalles gen. nov. (type species: Kyklioacalles nevadaensis Stuben, 2001), Coloracalles gen. nov. (type species: Acalles humerosus Fairmaire, 1862) and Elliptacalles gen. nov. (type species: Acalles longus Desbrochers, 1892). Relevant external characters and the male genitalia of all discussed taxa are illustrated. Three species are transferred to different genera: Kyklioacalles aubei (Boheman, 1837) (formerly: Acalles), Ruteria major (Solari A. & F., 1907) and Ruteria minosi (Bahr & Bayer, 2005) (both formerly Echinodera). VL - 22 SN - 1445-5226 ER -