01528nas a2200193   4500008004100000020001400041245009500055210006900150260000900219300001000228490000700238520086900245653002701114100002201141700002101163700002101184700001901205856011001224       2011                        eng d  a0021-491400aSimplification of the collecting method of adult sweetpotato weevils from artificial diets0 aSimplification of the collecting method of adult sweetpotato wee  c2011  a22-240 v553 aThe West Indian sweetpotato weevil Euscepes postfasciatus (Fairmaire) (Coleoptera: Curculionidae) is a major pest of the sweet potato Ipomoea batatas (L.) Lam (Solanales: Convolvulaceae) in some countries. In order to improve the mass-rearing systems involved in an eradication program employing the sterile insect technique (SIT), we improved the efficiency of the method used for collecting adult weevils from artificial larval diets; this was done by using the combination of a sieve and Tullgren apparatus. Using this combination, we could collect 95.4% of weevils from artificial diets. Furthermore, the time spent in collection with this method was approximately one-fifth of that spent with the existing hand-sorting method. These results showed that this new collection method will improve the efficiency of mass rearing of West Indian sweetpotato weevils.10aEuscepes postfasciatus1 aKuriwada, Takashi1 aKumano, Norikuni1 aShiromoto, Keiko1 aHaraguchi, Dai  uhttps://weevil.myspecies.info/simplification-collecting-method-adult-sweetpotato-weevils-artificial-diets01385nas a2200193   4500008004100000020001400041245013600055210006900191260000900260300001000269490000700279520066000286653002700946100002200973700002100995700002101016700001901037856013501056       2011                        eng d  a0021-491400aThe effect of an artificial adult diet on the reproductive performance of the West Indian sweetpotato weevil Euscepes postfasciatus0 aeffect of an artificial adult diet on the reproductive performan  c2011  a27-290 v553 aThe West Indian sweetpotato weevil Euscepes postfasciatus (Fairmaire) (Coleoptera: Curculionidae) is a major pest of the sweet potato Ipomoea batatas (L.) Lam (Solanales: Convolvulaceae) in some countries. To improve mass rearing systems involved in an eradication program employing the sterile insect technique (SIT), we examined the effect of an artificial adult diet on the reproductive performance of both male and female E. postfasciatus. The artificial diet had a negative effect on female fecundity but no effect on male mating ability. Our results suggest that the artificial diet should be improved to prevent adverse effects on female fecundity.10aEuscepes postfasciatus1 aKuriwada, Takashi1 aKumano, Norikuni1 aShiromoto, Keiko1 aHaraguchi, Dai  uhttps://weevil.myspecies.info/effect-artificial-adult-diet-reproductive-performance-west-indian-sweetpotato-weevil-euscepes-postfa01486nas a2200193   4500008004100000020001400041245012400055210006900179260000900248300001000257490000700267520078400274653002201058100002301080700001701103700001901120700001901139856013401158       2009                        eng d  a0021-491400aPCR-RFLP Discrimination between Okinawa and Ogasawara Strains of the Sweet Potato Weevil, Cylas formicarius (Fabricius)0 aPCRRFLP Discrimination between Okinawa and Ogasawara Strains of   c2009  a60-630 v533 aTo establish a simple method for discriminating Okinawa and Ogasawara strains of the sweet potato weevil, Cylas formicarius, about 560 bp of the internal transcribed spacer-1 (ITS-1) region of the ri- bosomal DNA (rDNA) of C. formicarius was collected from several is- lands of Okinawa Prefecture and Ogasawara Islands and analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The 345 weevils collected in Okinawa and Ogasawara were discriminated correctly by the RFLP patterns with the restriction enzyme RsaI. The results suggest that C. formicarius originating from Ogasawara Islands is an effective genetic marking strain for an eradica- tion project using the sterile insect technique implemented by the Oki- nawa prefectural government.10aCylas formicarius1 aKawamura, Kiyohisa1 aOhno, Suguru1 aHaraguchi, Dai1 aKohama, Tsuguo  uhttps://weevil.myspecies.info/pcr-rflp-discrimination-between-okinawa-and-ogasawara-strains-sweet-potato-weevil-cylas-formicarius