01466nas a2200169   4500008004100000020001400041245011800055210006900173260000800242300001200250490000700262520082200269653002701091100002301118700002001141856013501161       2010                        eng d  a0003-686200aNardonella endosymbiont in the West Indian sweet potato weevil Euscepes postfasciatus (Coleoptera: Curculionidae)0 aNardonella endosymbiont in the West Indian sweet potato weevil E  cFeb  a115-1200 v453 aThe West Indian sweet potato weevil, Euscepes postfasciatus, is a notorious pest of the sweet potato, Ipomoea batatas. We examined the potential presence of a bacterial endosymbiont in the pest weevil. The bacterial 16S rRNA gene and groEL gene were detected by PCR from the insects. Cloning, sequencing and molecular phylogenetic analyses of the bacterial genes demonstrated that E. postfasciatus is associated with a [gamma]-proteobacterial endosymbiont of the genus Nardonella. In situ hybridization detected the endosymbiont in the female ovaries, indicating its transovarial transmission through host generations. This study is the first to identify Nardonella from the weevil subfamily Cryptorhynchinae. The potential relevance of the endosymbiont in the biology and management of E. postfasciatus is discussed.10aEuscepes postfasciatus1 aHosokawa, Takahiro1 aFukatsu, Takema  uhttps://weevil.myspecies.info/nardonella-endosymbiont-west-indian-sweet-potato-weevil-euscepes-postfasciatus-coleoptera-curculioni01732nas a2200169   4500008004100000020001400041245019400055210006900249260000800318300001200326490000700338520101800345653002501363100002101388700001801409856013501427       2010                        eng d  a0003-686200aGenetic variation among Japanese populations of Platypus quercivorus (Coleoptera: Platypodidae), an insect vector of Japanese oak wilt disease, based on partial sequence of nuclear 28S rDNA0 aGenetic variation among Japanese populations of Platypus querciv  cMay  a319-3280 v453 aThe ambrosia beetle, Platypus quercivorus, is an insect vector of the Ascomycete fungus, Raffaelea quercivora, the causal agent of Japanese oak wilt disease. In this study, we compared the nucleotide sequences (937-955 bp) of the nuclear 28S rDNA fragment, including the D1 and D2 expansion segment, among local populations of the beetle in Japan. Sequence analysis of 81 beetles from 32 collection sites ranging from the Ryukyu Islands to northern Honshu Island showed seven types of sequences, many of which were separated among different geographic areas. Phylogenetic analysis showed that these sequence types were separated between two deeply divergent genetic groups, suggesting the necessity for taxonomic reexamination of this species. DNA typing of beetles and oak wilt status at the collection sites indicated that several genetically divergent beetle populations are associated with the incidence of the disease. This is the first investigation of genetic variation in the ambrosia beetle across Japan.10aPlatypus quercivorus1 aHamaguchi, Keiko1 aGoto, Hideaki  uhttps://weevil.myspecies.info/genetic-variation-among-japanese-populations-platypus-quercivorus-coleoptera-platypodidae-insect-vec