01898nas a2200169   4500008004100000020001400041245013800055210006900193260000800262300001400270490000800284520123200292653002401524100002301548700002201571856013501593       2010                        eng d  a0031-949X00aQuantification of Propagules of the Laurel Wilt Fungus and Other Mycangial Fungi from the Redbay Ambrosia Beetle, Xyleborus glabratus0 aQuantification of Propagules of the Laurel Wilt Fungus and Other  cOct  a1118-11230 v1003 aThe laurel wilt pathogen, Raffaelea lauricola, is a fungal symbiont of the redbay ambrosia beetle, Xyleborus glabratus. which is native to Asia and was believed to have brought R. lattricola with it to the southeastern United States. Individual X. glabratus beetles from six populations in South Carolina and Georgia were individually macerated in glass tissue grinders and serially diluted to quantify the CFU of fungal symbionts. Six species of Raffaelea were isolated, with up to four species from an individual adult beetle. The Raffae lea spp. were apparently within the protected, paired, mandibular mycangia because they were as numerous in heads as in whole beetles, and surface-sterilized heads or whole bodies yielded as many or more CFU as did nonsterilized heads or whole beetles. R. lauricola was isolated from 40 of the 41 beetles sampled, and it was isolated in the highest numbers, up to 30,000 CFU/beetle. Depending on the population sampled, R. subalba or R. ellipticospora was the next most frequently isolated species. R. arxii, R. fusca, and R. subfusca were only occasionally isolated. The laurel wilt pathogen apparently grows in a yeast phase within the mycangia in competition with other Raffaelea spp.10aXyleborus glabratus1 aHarrington, T., C.1 aFraedrich, S., W.  uhttps://weevil.myspecies.info/quantification-propagules-laurel-wilt-fungus-and-other-mycangial-fungi-redbay-ambrosia-beetle-xylebo