02821nas a2200325   4500008004100000020001400041245014800055210006900203260000800272300001200280490000700292520173200299653001502031100002902046700002202075700001402097700001502111700002002126700002302146700002502169700002002194700002202214700001702236700002002253700002102273700002102294700002502315700002002340856013502360       2012                        eng d  a0965-174800aTranscriptome and full-length cDNA resources for the mountain pine beetle, Dendroctonus ponderosae Hopkins, a major insect pest of pine forests0 aTranscriptome and fulllength cDNA resources for the mountain pin  cAug  a525-5360 v423 aBark beetles (Coleoptera: Curculionidae: Scolytinae) are major insect pests of many woody plants around the world. The mountain pine beetle (MPB), Dendroctonus ponderosae Hopkins, is a significant historical pest of western North American pine forests. It is currently devastating pine forests in western North America - particularly in British Columbia, Canada - and is beginning to expand its host range eastward into the Canadian boreal forest, which extends to the Atlantic coast of North America. Limited genomic resources are available for this and other bark beetle pests, restricting the use of genomics-based information to help monitor, predict, and manage the spread of these insects. To overcome these limitations, we generated comprehensive transcriptome resources from fourteen full-length enriched cDNA libraries through paired-end Sanger sequencing of 100,000 cDNA clones, and single-end Roche 454 pyrosequencing of three of these cDNA libraries. Hybrid de novo assembly of the 3.4 million sequences resulted in 20,571 isotigs in 14,410 isogroups and 246,848 singletons. In addition, over 2300 non-redundant full-length cDNA clones putatively containing complete open reading frames, including 47 cytochrome P450s, were sequenced fully to high quality. This first large-scale genomics resource for bark beetles provides the relevant sequence information for gene discovery; functional and population genomics; comparative analyses; and for future efforts to annotate the MPB genome. These resources permit the study of this beetle at the molecular level and will inform research in other Dendroctonus spp. and more generally in the Curculionidae and other Coleoptera. (C) 2012 Elsevier Ltd. All rights reserved.10aScolytinae1 aKeeling, Christopher, I.1 aHenderson, Hannah1 aLi, Maria1 aYuen, Mack1 aClark, Erin, L.1 aFraser, Jordie, D.1 aHuber, Dezene, P. W.1 aLiao, Nancy, Y.1 aDocking, Roderick1 aBirol, Inanc1 aChan, Simon, K.1 aTaylor, Greg, A.1 aPalmquist, Diana1 aJones, Steven, J. M.1 aBohlmann, Joerg  uhttps://weevil.myspecies.info/transcriptome-and-full-length-cdna-resources-mountain-pine-beetle-dendroctonus-ponderosae-hopkins-ma