02217nas a2200181   4500008004100000020001400041245011200055210006900167260000900236300001200245490000800257520155600265653003401821100002101855700001901876700001701895856012301912       2007                        eng d  a0022-049300aEffect of irradiation on dispersal ability of male sweetpotato weevils (Coleoptera: Brentidae) in the field0 aEffect of irradiation on dispersal ability of male sweetpotato w  cJune  a730-7360 v1003 aWe used the mark-and-recapture method in the field to test the effect of gamma radiation on the dispersal ability of the male sweetpotato weevil, Cylas formicarius elegantulus (Summers) (Coleoptera: Brentidae), a serious sweetpotato pest in Japan. To evaluate the dispersal ability of male sweetpotato weevil, we released 27,218 males (13,302 males irradiated with a dose of 200 Gy and 13,916 nonirradiated males) in two replications (September and October 2005). Each replication lasted 5 d from the release of weevils to the removal of traps, and male weevils were released twice (1 and 3 d before trap setting). Forty pheromone traps were placed in lines corresponding to eight compass directions and five distance classes (20,50,100,200, and 500 m) in each replication. We captured 2,263 irradiated males (17.0%) and 2,007 nonirradiated males (14.4%) in the two replications. Six irradiated and eight nonirradiated males were captured in the traps 500 m far from the release point. All parameters to evaluate the dispersal ability of irradiated male sweetpotato weevil (recapture rate, dispersal distance, and dispersal direction) were similar to nonirradiated males in three of the four trials. However, parameters were different between irradiated males and nonirradiated males in one trial. Because the majority of parameters consistently show that the similarity of the dispersal ability, we considered that male sweetpotato weevil irradiated with a dose of 200 Gy possessed equal dispersal ability to that of nonirradiated males in the field.10aCylas formicarius elegantulus1 aKumano, Norikuni1 aKohama, Tsuguo1 aOhno, Suguru  uhttps://weevil.myspecies.info/effect-irradiation-dispersal-ability-male-sweetpotato-weevils-coleoptera-brentidae-field01718nas a2200205   4500008004100000020001400041245013400055210006900189260000800258300001200266490000700278520097000285653002201255100002301277700001701300700001901317700002201336700001901358856013501377       2009                        eng d  a0003-686200aGeographic variation of elytral color in the sweet potato weevil, Cylas formicarius (Fabricius) (Coleoptera: Brentidae), in Japan0 aGeographic variation of elytral color in the sweet potato weevil  cNov  a505-5130 v443 aTo use elytral color morphs of Cylas formicarius as a genetic marker in its eradication using the sterile insect technique, the geographic distribution of the morphs was examined in the three main infested areas of Japan (Central Ryukyus, Southern Ryukyus, and Ogasawara Islands). In addition to the two known color morphs in Japan (bluish elytra (BE) and greenish elytra. (GE)), the piceous elytra (PE) was found mainly in the Southern Ryukyus at low frequency. The color morph frequencies varied between the Central and Southern Ryukyus and between locations within the two areas, while only BE was found in the Ogasawara Islands. PE would be useful for genetically marking sterile weevils to be released. Similarly, GE would be useful in some islands where GE is rarely or not found. BE frequencies on two islands in the Central Ryukyus (Amami and Okinawa) and two islands in the Southern Ryukyus (Miyako and Ishigaki) in 2006-2007 were higher than in 2002-2003.10aCylas formicarius1 aKawamura, Kiyohisa1 aOhno, Suguru1 aHaraguchi, Dai1 aKawashima, Shunji1 aKohama, Tsuguo  uhttps://weevil.myspecies.info/geographic-variation-elytral-color-sweet-potato-weevil-cylas-formicarius-fabricius-coleoptera-brenti01486nas a2200193   4500008004100000020001400041245012400055210006900179260000900248300001000257490000700267520078400274653002201058100002301080700001701103700001901120700001901139856013401158       2009                        eng d  a0021-491400aPCR-RFLP Discrimination between Okinawa and Ogasawara Strains of the Sweet Potato Weevil, Cylas formicarius (Fabricius)0 aPCRRFLP Discrimination between Okinawa and Ogasawara Strains of   c2009  a60-630 v533 aTo establish a simple method for discriminating Okinawa and Ogasawara strains of the sweet potato weevil, Cylas formicarius, about 560 bp of the internal transcribed spacer-1 (ITS-1) region of the ri- bosomal DNA (rDNA) of C. formicarius was collected from several is- lands of Okinawa Prefecture and Ogasawara Islands and analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The 345 weevils collected in Okinawa and Ogasawara were discriminated correctly by the RFLP patterns with the restriction enzyme RsaI. The results suggest that C. formicarius originating from Ogasawara Islands is an effective genetic marking strain for an eradica- tion project using the sterile insect technique implemented by the Oki- nawa prefectural government.10aCylas formicarius1 aKawamura, Kiyohisa1 aOhno, Suguru1 aHaraguchi, Dai1 aKohama, Tsuguo  uhttps://weevil.myspecies.info/pcr-rflp-discrimination-between-okinawa-and-ogasawara-strains-sweet-potato-weevil-cylas-formicarius