02077nas a2200181   4500008004100000020001400041245018300055210006900238260000900307300001200316490000800328520134500336653001701681100002301698700001801721700002101739856013501760       2010                        eng d  a0022-049300aMolecular Identification of Larval Stages of Otiorhynchus (Coleoptera: Curculionidae) Species Based on Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Analysis0 aMolecular Identification of Larval Stages of Otiorhynchus Coleop  cJune  a898-9070 v1033 aA couple of different members of the coleopteran genus Otiorhynchus (Coleoptera: Curculionidae) are becoming increasingly important as pests of nursery and ornamental plants in global horticulture. Although adult weevils are morphologically distinguishable by skilled personnel, high potential for misidentification is given for cryptic larval stages. For developing and applying efficient pest management strategies the determination of the respective species is however a prerequisite, because each species may have a different phenology or a varying susceptibility to pesticides. Here, we report on the development of a diagnostic polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) method for differentiation among 16 Otiorhynchus and seven other weevil species independent of their developmental stage. An [approximately]780-bp fragment of the mitochondrial cytochrome oxidase subunit II was amplified and subsequently digested with at most four restriction enzymes generating species-specific fragment patterns. The assay was validated on a total of 127 individuals and the obtained fragment patterns correctly identified 23 different weevil species. The PCR-RFLP method reported here is cost-effective, robust, and fast and could be used in the future by plant protection services for diagnostic purposes.10aOtiorhynchus1 aHirsch, Jacqueline1 aSprick, Peter1 aReineke, Annette  uhttps://weevil.myspecies.info/molecular-identification-larval-stages-otiorhynchus-coleoptera-curculionidae-species-based-polymeras01281nas a2200241   4500008004100000020002500041245014600066210006900212260001200281300001000293490000900303520045800312653001700770653002700787653002500814653002600839653003000865653002600895653002800921100001700949700001800966856005500984       2012                        eng d  a1175-5326; 1175-533400aMorphology and identification of the pupae of seven species of the genus Otiorhynchus Germar, 1822 (Coleoptera, Curculionidae, Otiorhynchini)0 aMorphology and identification of the pupae of seven species of t  c14 Sept  a39-570 v34833 aThe pupae of Otiorhynchus armadillo (Rossi, 1792), O. aurifer Boheman, 1843, O. crataegi Germar, 1824, O. meridionalis Gyllenhal, 1834, O. porcatus (Herbst, 1795) and O. salicicola Heyden, 1908, are described and illustrated for the first time. The pupa of O. sulcatus (Fabricius, 1775) is redescribed and illustrated. Characteristic features including chaetotaxy and habitus, measurements and a key to the species based on the pupae are also presented.10aOtiorhynchus10aOtiorhynchus armadillo10aOtiorhynchus aurifer10aOtiorhynchus crataegi10aOtiorhynchus meridionalis10aOtiorhynchus porcatus10aOtiorhynchus salicicola1 aGosik, Rafal1 aSprick, Peter  uhttp://www.mapress.com/zootaxa/list/2012/3483.html